>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
无创性延迟肢体缺血预适应对脑缺血再灌注大鼠存活素和血管内皮生长因子表达的影响
作者:鲁慧1  王彬成1  崔宁宁1  张艳春1  杨兰2 
单位:1. 沧州市中心医院 神经内科, 河北 沧州 061000;
2. 河北医科大学第二医院 神经内科, 河北 石家庄 053600
关键词:无创性延迟肢体缺血预适应 脑缺血再灌注 大鼠 存活素 血管内皮生长因子 
分类号:R743;R33-33
出版年·卷·期(页码):2018·37·第五期(797-801)
摘要:

目的:探讨无创性延迟肢体缺血预适应对脑缺血再灌注大鼠存活素和血管内皮生长因子(VEGF)表达的影响。方法:根据随机数字表法将90只大鼠分为假手术组(sham组)、脑缺血再灌注损伤组(I/R组)、脑缺血再灌注损伤+无创性延迟肢体缺血预适应组(I/R+NDLIP组),每组30只。测定各组大鼠神经缺损症状和行为评分、脑梗死范围,采用免疫组化二步法测定各组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞,采用Western blotting法测定存活素和VEGF蛋白表达。结果:sham组大鼠未出现神经缺损症状,I/R+NDLIP组大鼠神经缺损症状和行为评分低于I/R组(P<0.05)。sham组大鼠未见脑梗死区域,I/R+NDLIP组大鼠脑梗死范围明显小于I/R组(P<0.05)。I/R组和I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞表达均高于sham组(P<0.05),I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF阳性细胞表达均高于I/R组(P<0.05)。I/R组和I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF蛋白相对表达量均高于sham组(P<0.05),I/R+NDLIP组大鼠梗死灶周边皮质区存活素和VEGF蛋白相对表达量均高于I/R组(P<0.05)。结论:无创性延迟肢体缺血预适应可降低脑缺血再灌注对神经的损伤,减小脑梗死范围,其机制可能与无创性延迟肢体缺血预适应可上调梗死灶周边皮质区存活素和VEGF的表达有关。

Objective:To investigate the effects of noninvasive delayed limb ischemic preconditioning on expression of survivin and vascular endothelial growth factor(VEGF) in rats with cerebral ischemia-reperfusion.Methods:90 rats were divided into sham group, cerebral ischemia-reperfusion injury group (I/R group), cerebral ischemia-reperfusion injury + noninvasive delayed limb ischemic preconditioning group(I/R+NDLIP group) according to random number method, 30 rats in each group. The neurological deficits and behavior scores, cerebral infarction were detected.Two-step immunohistochemical method was used to detect the survival rate and VEGF-positive cells in the cortex of the infarct.The expression of survivin and VEGF protein were detected by Western blotting. Results:No neurological deficits occured in rats of sham group, the scores of neurological deficits and behavioral in I/R+NDLIP group were lower than those in I/R group (P<0.05).The sham group had no cerebral infarction area,the cerebral infarction in I/R + NDLIP group was significantly smaller than that in I/R group (P<0.05).The survivin and VEGF positive cells in the cortex in the I/R group and the I/R + NDLIP group were significantly higher than those in the sham group (P<0.05).The survivin and VEGF positive cells in the cortex in the I/R + NDLIP group were higher than those in the I/R group (P<0.05).The relative expression of survivin and VEGF protein in the cortex of the I/R group and the I/R + NDLIP group were higher than those in the sham group (P<0.05).The relative expression of survivin and VEGF protein in the cortex of the infarct in the I/R + NDLIP group were significantly higher than those in the I/R group (P<0.05).Conclusion:Noninvasive delayed limb ischemic preconditioning can reduce cerebral ischemia and reperfusion injury to the nerve,reduce the scope of cerebral infarction,its mechanism may be related to noninvasive delayed limb ischemic preconditioning can upregulate the expression of survivin and VEGF in the cortex of the infarct.

参考文献:

[1] JABBARLI R,REINHARD M,NIESEN W D,et al.Predictors and impact of early cerebral infarction after aneurysmal subarachnoid hemorrhage[J].Eur J Neurol,2015,22(6):941-947.
[2] HUANG H L,WANG N,ZHOU H,et al.Study on influence of transient ischemic attack on subsequent cerebral infarction[J].Eur Rev Med Pharmacol Sci,2016,20(24):5164-5167.
[3] WANG W,YU X D,MO X,et al.Limb ischemic preconditioning attenuates cerebral ischemic injury in rat model[J].Perfusion,2014,29(3):210-218.
[4] 陈江龙,张灏龙,赵渝,等.存活素基因过表达对血管内皮细胞迁移和增殖能力的影响[J].上海交通大学学报(医学版),2016,36(4):469-473,480.
[5] RAMCHANDANI D,WEBER G F.Interactions between osteopontin and vascular endothelial growth factor:Implications for cancer[J].Biochim Biophys Acta,2015,1855(2):202-222.
[6] WANG Z,JI Y,WANG S,et al.Protective effect of intestinal ischemic preconditioning on ischemia reperfusion-caused lung injury in rats[J].Inflammation,2015,38(1):424-432.
[7] 王洪志,张一娜,武小薇.远隔缺血预适应对大鼠心肌缺血再灌注的保护作用[J].中西医结合心脑血管病杂志,2015,13(12):1378-1380.
[8] KAGEYAMA S,HATA K,TANAKA H,et al.Intestinal ischemic preconditioning ameliorates hepatic ischemia/reperfusion injury in rats:role of heme oxygenase 1 in the second window of protection[J].Liver Transpl,2015,21(1):112-122.
[9] 闫峰,尹洁,罗玉敏,等.远隔缺血预适应对大鼠局灶性脑缺血后诱导型一氧化氮合酶的影响[J].首都医科大学学报,2014,35(3):315-319.
[10] TOTZECK M,HENDGEN-COTTA U,RASSAF T.Concepts of hypoxic NO signaling in remote ischemic preconditioning[J].World J Cardiol,2015,7(10):645-651.
[11] LI S,MA C,SHAO G,et al.Safety and Feasibility of Remote Limb Ischemic Preconditioning in Patients With Unilateral Middle Cerebral Artery Stenosis and Healthy Volunteers[J].Cell Transplant,2015,24(9):1901-1911.
[12] 阮溦,刘青,陈婵,等.自噬通过PPAR-γ介导肢体远隔缺血预适应减少肝缺血再灌注损伤(英文)[J].中南大学学报(医学版),2016,41(9):918-928.
[13] DENG Q W,XIA Z Q,QIU Y X,et al.Clinical benefits of aortic cross-clamping versus limb remote ischemic preconditioning in coronary artery bypass grafting with cardiopulmonary bypass:a meta-analysis of randomized controlled trials[J].J Surg Res,2015,193(1):52-68.
[14] JAISWAL P K,GOEL A,MITTAL R D.Survivin:A molecular biomarker in cancer[J].Indian J Med Res,2015,141(4):389-397.
[15] 林凤,王芳,周凯.子宫内膜癌中Survivin与EGFR的表达及其临床意义[J].温州医科大学学报,2016,46(8):594-596.
[16] ZHANG L,YAN R,ZHANG Q,et al.Survivin,a key component of the Wnt/β-catenin signaling pathway,contributes to traumatic brain injury-induced adult neurogenesis in the mouse dentate gyrus[J].Int J Mol Med,2013,32(4):867-875.
[17] SARASWAT D,NEHRA S,CHAUDHARY K,et al.Novel vascular endothelial growth factor blocker improves cellular viability and reduces hypobaric hypoxia-induced vascular leakage and oedema in rat brain[J].Clin Exp Pharmacol Physiol,2015,42(5):475-484.
[18] OZBEK E,ADAS G,OTUNCTEMUR A,et al.Role of Mesenchymal Stem Cells Transfected With Vascular Endothelial Growth Factor in Maintaining Renal Structure and Function in Rats with Unilateral Ureteral Obstruction[J].Exp Clin Transplant,2015,13(3):262-272.
[19] 董桂英.高压氧治疗急性脑梗死的疗效以及对患者血清血管内皮生长因子、促血管生成素Ⅱ的影响[J].中国现代医学杂志,2016,26(14):52-57.

服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 152988 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-87232483 83272481
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364