紫杉醇联合顺铂调控MAPK/NF-κB信号治疗幽门螺杆菌胃癌机制的研究-东南大学学报医学版

紫杉醇联合顺铂调控MAPK/NF-κB信号治疗幽门螺杆菌胃癌机制的研究

单位：南通市第一人民医院肿瘤内科, 江苏南通 226000

分类号：R735.2;R730.53

出版年·卷·期（页码）：2018·37·第四期（706-710）

摘要：

目的：探讨紫杉醇联合顺铂对幽门螺杆菌胃癌的治疗作用，及丝裂原活化蛋白激酶（mitogen-activated protein kinase，MAPK）/核转录因子kappa B（nuclear factor kappa-light-chain-enhancer of activated B cells，NF-κB）信号通路在幽门螺杆菌胃癌患者中的表达变化及意义。方法：给予幽门螺杆菌胃癌患者紫杉醇联合顺铂化疗2个疗程，采用免疫组织化学法检测胃癌患者癌组织中MAPK和NF-κB蛋白的表达；采用Real-Time PCR法检测胃癌组织中MAPK和NF-κB mRNA的表达；采用Western blotting检测胃癌组织中凋亡蛋白MAPK和NF-κB表达；ELISA法检测患者p65和Caspase 3含量。结果：与治疗前相比，治疗后胃癌患者癌组织中的MAPK和NF-κB蛋白表达及mRNA表达水平显著升高，凋亡蛋白p65和Caspase 3表达也显著性增加，差异均有统计学意义（P<0.05）。结论：紫杉醇联合顺铂对幽门螺杆菌胃癌有一定的治疗作用，且MAPK/NF-κB信号通路在此过程中发挥重要的作用。

Objective:To investigate the therapeutic effect of paclitaxel combined with cisplatin on Helicobacter pylori gastric cancer, and the significance of MAPK/NF-κB signaling pathway in patients with Helicobacter pylori (Hp) -related gastric cancer. Methods:Helicobacter pylori gastric cancer patients given paclitaxel combined with cisplatin chemotherapy 2 course of treatment, and immunohistochemical method was used to detect the expression of MAPK and NF-κB protein in cancer tissues of patients with gastric cancer. The expressions of MAPK and NF-κB mRNA in gastric carcinoma were detected by real-time PCR. Western blotting was used to detect the expression of apoptotic protein MAPK and NF-κB in gastric carcinoma. The levels of p65 and Caspase 3 were detected by ELISA. Results:Compared with before treatment, the expression of MAPK and NF-κB protein and mRNA in cancer tissues of patients with gastric cancer were significantly increased after treatment. The expression of apoptotic protein p65 and Caspase 3 also increased significantly, the difference was statistically significant (P<0.05). Conclusion:Paclitaxel combined with cisplatin has a certain therapeutic effect on Helicobacter pylori gastric cancer. And MAPK/NF-κB signaling pathway plays an important role in this process.

参考文献：

[1] 路滟,徐耀初,沈洪兵.NOD1和NOD2基因多态性与胃癌遗传易感性的研究[J].中华疾病控制杂志,2011,15(9):735-739.
[2] 胡立民.幽门螺杆菌的致病机制[J].临床军医杂志,2004,32(6):117-118.
[3] 李唯琼,张超.幽门螺杆菌与胃癌相关性探讨[J].当代医学,2010,16(32):41-42.
[4] 唐寅思,曹蓉,翁洋,等.幽门螺杆菌阳性患者胃黏膜组织中NOD1受体的表达变化[J].山东医药,2016,56(21):79-81.
[5] KOCH K N,MÜLLER A. Helicobacter pylori activates the TLR2/NLRP3/caspase-1/IL-18 axis to induce regulatory T-cells,establish persistent infection and promote tolerance to allergens[J].Gut Microbes,2015,6(6):382-387.
[6] 许先荣,赵桂芝,朱婉萍,等. 紫杉醇脂质体联合中药姜黄素对小鼠转移性肺癌的影响[J].中国中医药科技,2016,23(5):551-553.
[7] 任维维,米登海,李征,等.非小细胞肺癌紫杉醇类联合顺铂同步或序贯放化疗对比的Meta分析[J].中华肿瘤防治杂志,2013,20(5):337-382.
[8] CHUA C,TAN I B,YAMADA Y,et al. Phase Ⅱ study of trastuzumab in combination with S-1 and cisplatin in the first-line treatment of human epidermal growth factor receptor HER2-positive advanced gastric cancer[J].Cancer Chemoth Pharm,2015,76(2):397-408.
[9] MITSUI Y,SATO Y,MIYAMOTO H,et al. Trastuzumab in combination with docetaxel/cisplatin/S-1(DCS) for patients with HER2-positive metastatic gastric cancer:feasibility and preliminary efficacy[J].Cancer Chemoth Pharm,2015,76(2):375-382.
[10] 郁迪,莫绪明.PI3K/Akt和MAPK信号通路在缺血性脑损伤中的保护作用[J].医学综述,2015,21(2):210-213.
[11] ZHANG X,MA L,QI J,et al.MAPK/ERK signaling pathway-induced hyper-O-GlcNAcylation enhances cancer malignancy[J].Mol Cell Biochem,2015,410(1-2):101-110.
[12] 许爱霞,郑莉萍,唐丹凤,等. MAPK/PKC信号通路在原癌基因c-erbB₂调控原始卵泡启动生长中的作用[J].生理学报,2009,61(5):439-444.
[13] 黄小平,卢金冬,丁煌,等.黄芪和三七的主要有效成分配伍对脑缺血/再灌注小鼠NF-κB信号通路及炎性因子表达的影响[J].中国药理学通报,2015,31(1):141-146.
[14] 隋欣,孙纲,关宏伟,等.人胃腺癌p38MAPK信号通路表达规律与多药耐药相关因子、病理情况及化疗后预后的相关性研究[J].现代医学,2017,45(2):256-259.
[15] 隋华,付晓伶,潘树芳,等.PI3K/Akt/NF-κB通路调控ABCB1/P-gp介导的人结肠癌细胞多药耐药的研究[J].中国癌症杂志,2015,24(2):106-111.

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