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紫杉醇联合顺铂调控MAPK/NF-κB信号治疗幽门螺杆菌胃癌机制的研究
作者:缪亚军  杨莉 
单位:南通市第一人民医院 肿瘤内科, 江苏 南通 226000
关键词:幽门螺杆菌 胃癌 丝裂原活化蛋白激酶 核转录因子kappa B 紫杉醇 顺铂 
分类号:R735.2;R730.53
出版年·卷·期(页码):2018·37·第四期(706-710)
摘要:

目的:探讨紫杉醇联合顺铂对幽门螺杆菌胃癌的治疗作用,及丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)/核转录因子kappa B(nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)信号通路在幽门螺杆菌胃癌患者中的表达变化及意义。方法:给予幽门螺杆菌胃癌患者紫杉醇联合顺铂化疗2个疗程,采用免疫组织化学法检测胃癌患者癌组织中MAPK和NF-κB蛋白的表达;采用Real-Time PCR法检测胃癌组织中MAPK和NF-κB mRNA的表达;采用Western blotting检测胃癌组织中凋亡蛋白MAPK和NF-κB表达;ELISA法检测患者p65和Caspase 3含量。结果:与治疗前相比,治疗后胃癌患者癌组织中的MAPK和NF-κB蛋白表达及mRNA表达水平显著升高,凋亡蛋白p65和Caspase 3表达也显著性增加,差异均有统计学意义(P<0.05)。结论:紫杉醇联合顺铂对幽门螺杆菌胃癌有一定的治疗作用,且MAPK/NF-κB信号通路在此过程中发挥重要的作用。

Objective:To investigate the therapeutic effect of paclitaxel combined with cisplatin on Helicobacter pylori gastric cancer, and the significance of MAPK/NF-κB signaling pathway in patients with Helicobacter pylori (Hp) -related gastric cancer. Methods:Helicobacter pylori gastric cancer patients given paclitaxel combined with cisplatin chemotherapy 2 course of treatment, and immunohistochemical method was used to detect the expression of MAPK and NF-κB protein in cancer tissues of patients with gastric cancer. The expressions of MAPK and NF-κB mRNA in gastric carcinoma were detected by real-time PCR. Western blotting was used to detect the expression of apoptotic protein MAPK and NF-κB in gastric carcinoma. The levels of p65 and Caspase 3 were detected by ELISA. Results:Compared with before treatment, the expression of MAPK and NF-κB protein and mRNA in cancer tissues of patients with gastric cancer were significantly increased after treatment. The expression of apoptotic protein p65 and Caspase 3 also increased significantly, the difference was statistically significant (P<0.05). Conclusion:Paclitaxel combined with cisplatin has a certain therapeutic effect on Helicobacter pylori gastric cancer. And MAPK/NF-κB signaling pathway plays an important role in this process.

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