槐耳清膏抑制MCF-7细胞裸鼠成瘤性实验研究-东南大学学报医学版

槐耳清膏抑制MCF-7细胞裸鼠成瘤性实验研究

单位：1. 三峡大学第一临床医学院肿瘤防治中心, 宜昌市中心人民医院肿瘤科, 湖北宜昌 443000;
2. 武汉市中心医院武汉市肿瘤研究所, 湖北武汉 430061

分类号：R285.6

出版年·卷·期（页码）：2018·37·第一期（18-21）

摘要：

目的：构建裸鼠异种乳腺癌移植瘤模型（裸鼠接种MCF-7细胞），给予该模型鼠槐耳清膏处理，观察移植瘤组织病理变化特点，探讨其对裸鼠致瘤性的影响。方法：将裸鼠随机分为实验组和对照组，分别给予槐耳清膏（浓度150 mg·ml^-1）和0.9%生理盐水各0.2 ml灌胃，每天两次，共30 d。观察裸鼠瘤体积及瘤体重的变化，显微镜观察移植瘤组织病理变化特点。结果：裸鼠在接种MCF-7细胞3周后，接种部位均长出肿瘤，成瘤率100%，实验组和对照组移植瘤重分别为（0.20±0.07）g和（0.57±0.32）g，实验组裸鼠移植瘤体重明显低于对照组，差异具有统计学意义（P < 0.01），两组移植瘤体积分别为（0.32±0.74）cm³与（0.84±0.62）cm³，实验组移植瘤体积较对照组小，具有统计学差异（P < 0.01）。HE染色后，实验组裸鼠移植瘤镜下可见肿瘤组织细胞出现不同程度的退行性变，中央及边缘细胞出现大片缺血坏死区，在血供较丰富的区域表现尤为明显，而在坏死灶周围可见残存的肿瘤细胞。对照组细胞生长旺盛，有异型性，形态不规则，大小不等，细胞核大、浓染，可见病理性核分裂相，无明显坏死区。结论：在裸鼠体内，槐耳清膏能抑制乳腺癌细胞MCF-7的成瘤性，从而达到抑制乳腺癌细胞生长的作用。

Objective: The xenograft of breast cancer model in nude mice was reproduced to investigate the influences of Huaier cream on carcinogenicity in nude mice transplanted with MCF-7, so as to provide theoretical basis for clinical treatment. Methods: Nude mice were randomly divided into experimental group and control group, 4 mice in each group. Mice of the experimental group were lavaged with 0.2 mL of Huaier cream (150 mg·mL^-1), twice a day for a total of 30 days. The control group was given lavage with 0.2 mL of 0.9% saline, twice a day for a total of 30 days. The changes of tumor size and weights in nude mice were observed, the changes of pathological characteristics of transplanted tumor were observed under microscope. Results: In nude mouse tumorigenicity assay, each nude mouse developed tumor at the transplant site 3 weeks after inoculation with MCF-7 cells, with a tumor formation rate of 100%. The transplant tumors were (0.20±0.07)g and(0.57±0.32)g in the experimental groups after the experiment. And the transplant tumor was significantly lighter in the experimental groups than that of the control group, with statistically significant differences (P<0.01). Tumor volumes were (0.32±0.74)cm³ and (0.84±0.62)cm³, and those of the experimental groups were smaller than those of the control group, with statistically significance differences (P<0.01). HE staining showed in the nude mice of the experimental groups, there were different degrees of degeneration in the tumor cells, and large necrotic area in the central and the margin cells, especially in the area rich in blood supply, and residual tumor cells were visible around the necrosis focal; while in the control group, the cells were well grown, with atypia, in irregular shape and size, and the nuclei were large and densely dyed, pathological karyokinesis phase was seen, but no obvious necrotic area was found. Conclusion: In nude mice, Huaier cream can inhibit the tumor formation of MCF-7 cells, possibly due to the function of Huaier cream inhibiting the growth of breast cancer cells.

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