Objective: To explore the effect of H2S on liver injury caused by acetaminophen (APAP). Methods: Male C57BL/6J mice(n=20)were divided randomly into control group, APAP group, APAP+H2S group and H2S group. Eight hours they were treated with APAP, serum biochemical levels, the changes of morphological structure and the expression of related proteins were tested in all the groups. Results: After APAP treated, the level of ALT, AST were increased and massive necrosis could be observed under the pathologic microscope. Compared with APAP group, mice had lower ALT and AST, shown less tissue necrosis after H2S treatment. Meanwhile, APAP increased the expression of apoptotic proteins Cleaved-Caspase3 and Bax, inhibited the expression of Bcl-2 and activated MAPK/JNK signaling pathway. After H2S treatment, the expression of Cleaved-Caspase3 and Bax were reduced, Bcl-2 was increased and MAPK/JNK signaling pathway was supreessed. Conclusion: H2S can attenuate the APAP induced liver damage by inhibiting MAPK/JNK signaling pathway. |
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