Objective: To explore the effect of H2S on liver injury caused by acetaminophen (APAP). Methods: Male C57BL/6J mice(n=20)were divided randomly into control group, APAP group, APAP+H2S group and H2S group. Eight hours they were treated with APAP, serum biochemical levels, the changes of morphological structure and the expression of related proteins were tested in all the groups. Results: After APAP treated, the level of ALT, AST were increased and massive necrosis could be observed under the pathologic microscope. Compared with APAP group, mice had lower ALT and AST, shown less tissue necrosis after H2S treatment. Meanwhile, APAP increased the expression of apoptotic proteins Cleaved-Caspase3 and Bax, inhibited the expression of Bcl-2 and activated MAPK/JNK signaling pathway. After H2S treatment, the expression of Cleaved-Caspase3 and Bax were reduced, Bcl-2 was increased and MAPK/JNK signaling pathway was supreessed. Conclusion: H2S can attenuate the APAP induced liver damage by inhibiting MAPK/JNK signaling pathway.
 OSTAPOWICZ G,FONTANA R J,SCHIØDT F V,et al.Results of a prospective study of acute liver failure at 17 tertiary care centers in the United States[J].Ann Intern Med,2002,137(12):947-54.
 GUNAWAN B K,LIU Z X,HAN D,et.al.c-Jun N-terminal kinase plays a major role in murine acetaminophen hepatotoxicity[J].Gastroenterology,2006,131(1):165-78.
 LIU F C,LEE H C,LIAO C C,et.al.Tropisetron protects against acetaminophen-induced liver injury via suppressing hepatic oxidative stress and modulating the activation of jnk/erk mapk pathways[J].Biomed Res Int,2016(1):1952947
 兰爱平,梅卫义,孟金兰,等.硫化氢通过抑制p38 MAPK保护PC12细胞对抗化学性缺氧损伤[J].中国药理学通报,2010,26(10):1339-1344.
 ZHOU X,ZHAO L,MAO J,et.al.Antioxidant effects of hydrogen sulfide on left ventricular remodeling in smoking rats are mediated via PI3K/Akt-dependent activation of Nrf2[J].Toxicol Sci,2015,144(1):197-203.
 SAKAMOTO K,SUZUKI Y,KURAUCHI Y,et al.Hydrogen sulfide attenuates NMDA-induced neuronal injury via its anti-oxidative activity in the rat retina[J].Exp Eye Res,2014,120:90-96.
 TASLIPINAR M Y,AYDIN I,KALDIRIM U,et al.Hyperbaric oxygen treatment and N-acetylcysteine ameliorate acetaminophen-induced liver injury in a rat model[J].Hum Exp Toxicol,2013,32(10):1107-16.
 I·ÇER M,ZENGIN Y,GUNDUZ E,et al.Is montelukast as effective as N-acetylcysteine in hepatic injury due to acetaminophen intoxication in rats[J].Exp Toxicol Pathol,2016,68(1):55-59.
 WANG R.Two's company,three's a crowd:can H2S be the third endogenous gaseous transmitter[J].FASEB J,2002,16(13):1792-1798.
 JOHANSEN D,YTREHUS K,BAXTER GF.Exogenous hydrogen sulfide (H2S) protects against regional myocardial ischemia-reperfusion injury——Evidence for a role of K ATP channels[J].Basic Res Cardiol,2006,101(1):53-60.
 XU W,CHEN J,LIN J,et al.Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-κB and IL-1β pathways[J].Int J Mol Med,2015,35(1):177-186.
 LIU M H,LIN X L,ZHANG Y,et al.Hydrogen sulfide attenuates doxorubicin-induced cardiotoxicity by inhibiting reactive oxygen species-activated extracellular signal-regulated kinase 1/2 in H9c2 cardiac myocytes[J].Mol Med Rep,2015,12(5):6841-6848.