葛根不同萃取部位对链脲佐菌素致2型糖尿病大鼠糖脂代谢影响的研究-东南大学学报医学版

葛根不同萃取部位对链脲佐菌素致2型糖尿病大鼠糖脂代谢影响的研究

单位：1. 华中科技大学同济医学院附属武汉中心医院内分泌科, 湖北武汉 430000;
2. 武汉市第一医院骨外科, 湖北武汉 430022

分类号：R285.5;R587.1

出版年·卷·期（页码）：2017·36·第三期（356-360）

摘要：

目的：通过葛根不同萃取部位对链脲佐菌素（STZ）诱导的2型糖尿病大鼠糖脂代谢影响的研究，探索葛根降血糖的主要有效部位。方法：SD雄性大鼠高脂高糖饲料喂养1个月后，腹腔注射STZ 30 mg·kg^-1，建立2型糖尿病大鼠模型。将模型大鼠随机分为模型组、盐酸二甲双胍组（0.11 g·kg^-1）及葛根的萃余部位组、乙酸乙酯部位组、二氯甲烷部位组和石油醚部位组，连续灌胃给药4周。于给药后每周测定大鼠空腹血糖（FBG）值，眼眶后静脉丛取血测定糖化血红蛋白（HbA1c）、空腹血清胰岛素（FINS）水平，计算稳态胰岛素抵抗（HOMA-IR）指数和胰岛敏感指数（ISI），并对血清中的甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、游离脂肪酸（FFA）水平进行测定。结果：葛根二氯甲烷部位能明显降低糖尿病模型大鼠FBG、HbA1c、FINS、HOMA-IR指数、TG、TC、LDL-C、FFA，升高ISI。结论：葛根二氯甲烷部位、乙酸乙酯部位和萃余部位对2型糖尿病大鼠糖脂代谢具有较好的改善作用，其中以二氯甲烷部位作用最强。

Objective:To explore the effect of different extraction parts of Radix Puerarin on glucose and lipid metabolism in type 2 diabetic rats induced by STZ. Methods:SD male rats were fed with high fat and high sugar diet. After one month,the intraperitoneal injection of STZ 30 mg·kg^-1 was conducted and type 2 diabetic rat model was established. The model rats were randomly divided into model group,metformin group(0.11 g·kg^-1), Radix Puerariae raffinate site group,ethyl acetate group,dichloromethane fraction group and petroleum ether group,and the rats were continuously given intragastric administration for 4 weeks. The levels of fasting blood glucose(FBG), glycosylated hemoglobin(HbA1c)and fasting serum insulin(fins)were determined weekly and the steady-state insulin resistance(HOMA-IR)index and insulin sensitivity index(ISI)were calculated. The levels of serum triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C)and free fatty acid(FFA)were also measured. Results:Dichloromethane fraction of Radix Puerarin significantly decreased the levels of FBG,HbA1c,FPG,fins,HOMA-IR,TG,TC,LDL-C and FFA,and increased the ISI. Conclusion:Dichloromethane and ethylacetate extract, and raffinate parts of Puerarin can improve glucose and lipid metabolism in type 2 diabetic rats,of which the dichloromethane extract shows the best effect.

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