Objective: To investigate the effect of high level deoxycholic acid(DCA)on the IL-1β production and intestinal inflammation occurrence. Methods: Mice were fed with routine diet supplemented with 0.2% DCA, HE staining was performed to observe intestinal pathological injury; Western Blot and Realtime PCR were adopted to detect mature IL-1β production and IL-6, MCP-1 expression respectively; intestinal inflammation was assessed by the measurement of MPO activity. Results: Mice fed the DCA-supplemented diet developed obvious intestinal inflammation and injury, as evidenced by significant shortening of colon length [control group(8.1±0.5) cm vs DCA group(7.3±0.3)cm,P<0.01] and much higher MPO activity [control group(1.6±0.4)U·g-1 vs DCA group(3.0±0.5)U·g-1,P<0.01]; HE staining of colonic tissue in DCA group showed intestinal mucosal impairment, submucosal edema and inflammatory cell infiltration. Of note, the mature IL-1β level in colon tissue was elevated dramatically in DCA-fed group. Meanwhile, the mRNA levels of IL-6 (control group 1.0±0.1 vs DCA group 174.6±45.9,P<0.01) and MCP-1 (control group 1.0±0.1 vs DCA group 187.8±26.2,P<0.001) in DCA group were significantly increased as well. Conclusion: High level DCA can induce the occurrence of intestinal inflammation, which may at least partially depend on the activation of inflammasome and production of IL-1β. |
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