Objective: To investigate the effect of high level deoxycholic acid(DCA)on the IL-1β production and intestinal inflammation occurrence. Methods: Mice were fed with routine diet supplemented with 0.2% DCA, HE staining was performed to observe intestinal pathological injury; Western Blot and Realtime PCR were adopted to detect mature IL-1β production and IL-6, MCP-1 expression respectively; intestinal inflammation was assessed by the measurement of MPO activity. Results: Mice fed the DCA-supplemented diet developed obvious intestinal inflammation and injury, as evidenced by significant shortening of colon length [control group(8.1±0.5) cm vs DCA group(7.3±0.3)cm,P<0.01] and much higher MPO activity [control group(1.6±0.4)U·g-1 vs DCA group(3.0±0.5)U·g-1,P＜0.01]; HE staining of colonic tissue in DCA group showed intestinal mucosal impairment, submucosal edema and inflammatory cell infiltration. Of note, the mature IL-1β level in colon tissue was elevated dramatically in DCA-fed group. Meanwhile, the mRNA levels of IL-6 (control group 1.0±0.1 vs DCA group 174.6±45.9,P<0.01) and MCP-1 (control group 1.0±0.1 vs DCA group 187.8±26.2,P<0.001) in DCA group were significantly increased as well. Conclusion: High level DCA can induce the occurrence of intestinal inflammation, which may at least partially depend on the activation of inflammasome and production of IL-1β.
 KAPLAN G G.The global burden of IBD:from 2015 to 2025[J].Nat Rev Gastroenterol Hepatol,2015,12(12):720-727.
 FROLKIS A,DIELEMAN L A,BARKEMA H W,et al.Environment and the inflammatory bowel diseases[J].Can J Gastroenterol,2013,27(3):e18-e24.
 STENMAN L K,HOLMA R,KORPELA R.High-fat-induced intestinal permeability dysfunction associated with altered fecal bile acids[J].World J Gastroenterol,2012,18(9):923-929.
 RAIMONDI F,SANTORO P,BARONE M V,et al.Bile acids modulate tight junction structure and barrier function of Caco-2 monolayers via EGFR activation[J].Am J Physiol Gastrointest Liver Physiol,2008,294(4):G906-G913.
 BERNSTEIN H,HOLUBEC H,BERNSTEIN C,et al.Unique dietary-related mouse model of colitis[J].Inflamm Bowel Dis,2006,12(4):278-293.
 BAUER C,DUEWELL P,MAYER C,et al.Colitis induced in mice with dextran sulfate sodium (DSS) is mediated by the NLRP3 inflammasome[J].Gut,2010,59(9):1192-1199.
 SIEGMUND B,LEHR H A,FANTUZZI G,et al.IL-1 beta-converting enzyme (caspase-1) in intestinal inflammation[J].Proc Natl Acad Sci U S A,2001,98(23):13249-13254.
 SCHRODER K,TSCHOPP J.The inflammasomes[J].Cell,2010,140(6):821-832.
 COCCIA M,HARRISON O J,SCHIERING C,et al.IL-1beta mediates chronic intestinal inflammation by promoting the accumulation of IL-17A secreting innate lymphoid cells and CD4(+) Th17 cells[J].J Exp Med,2012,209(9):1595-1609.
 PAVLIDIS P,POWELL N,VINCENT R P,et al.Systematic review:bile acids and intestinal inflammation-luminal aggressors or regulators of mucosal defence?[J].Aliment Pharmacol Ther,2015,42(7):802-817.
 ALBERTS D S,EINSPAHR J G,EARNEST D L,et al.Fecal bile acid concentrations in a subpopulation of the wheat bran fiber colon polyp trial[J].Cancer Epidemiol Biomarkers Prev,2003,12(3):197-200.
 TRAUB R J,TANG B,JI Y,et al.A rat model of chronic postinflammatory visceral pain induced by deoxycholic acid[J].Gastroenterology,2008,135(6):2075-2083.
 CASTRO J,OCAMPO Y,FRANCO L.Cape gooseberry [Physalis peruviana L.] calyces ameliorate TNBS acid-induced colitis in rats[J].J Crohns Colitis,2015,9(11):1004-1015.
 KEATING N,KEELY S J.Bile acids in regulation of intestinal physiology[J].Curr Gastroenterol Rep,2009,11(5):375-382.