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肺癌细胞中NKG2D配体MICA及ULBP高表达及其在CIK介导的肿瘤细胞杀伤中的作用
作者:殷小伟1  卢绪章2  毛正道1  张倩1  曹琦1  黄燕华1 
单位:1. 南京医科大学附属常州第二人民医院 呼吸科, 江苏 常州 213161;
2. 南京医科大学附属常州第二人民医院 血液科, 江苏 常州 213161
关键词:肺癌 NKG2D配体 CIK细胞 MICA/B 
分类号:R734.2
出版年·卷·期(页码):2016·35·第六期(861-865)
摘要:

目的:探索肺癌细胞中NKG2D配体的表达量及其与CIK细胞介导的肿瘤细胞毒性的关系。方法:在肺癌组织、癌旁组织及肺癌细胞株中用实时荧光定量PCR及蛋白质免疫印迹法检测NKG2D配体的表达量。应用流式细胞仪检测肿瘤细胞株A549和QG56表面NKG2D配体的表达情况。体外分离培养CIK细胞,比较NKG2D单克隆抗体预处理CIK细胞和无NKG2D单克隆抗体预处理CIK细胞介导的肿瘤细胞毒性。结果:NKG2D在肺癌组织及肺癌细胞株中高表达。CIK细胞对肺癌细胞株A549表现出较强的细胞毒性,但用NKG2D单克隆抗体预处理CIK细胞后可显著降低这种作用(P<0.05)。结论:NKG2D配体在肺癌组织和肺癌细胞株中高表达。对于CIK细胞介导的肺癌细胞杀伤作用,NKG2D与其配体的相互作用至关重要。

Objective: To identify the expression level of NKG2D ligands in lung cancer cell and the interaction between NKG2D and NKG2D ligands in the CIK mediated cytotoxicity against tumor cells. Methods: We used RT-PCR and Western-blot to detect the expression level of NKG2D ligands in lung cancer tissue,para-carcinoma tissues and cell lines.The expression of NKG2D ligands in the surface of lung cancer cell lines was determined by flow cytometry.CIK cells were isolated and cultured in vitro.The anti-NKG2D mAbs treated CIK and non-anti-NKG2D mAbs treated CIK cells mediating cytotoxicity against A549 was determined by flow cytometry also. Results: NKG2D ligands highly expressed in lung cancer cells.The CIK cells caused cytolysis against the A549,but this cytolysis was decreased(30%±3.2%) by pretreatment of CIK cells with anti-NKG2D mAbs. Conclusion: The present study demonstrates the higher expression level of NKG2D ligands in lung cancer tissue than para-carcinoma tissue.The killing effect of lung cancer cells by CIK cell is partially mediated by NKG2D-NKG2D ligand interaction.The interaction between NKG2D and NKG2D ligands play a vital role in the CIK mediated tumor cell killing.

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