碱性成纤维细胞生长因子预处理对大鼠脑缺血/再灌注损伤的神经保护作用-东南大学学报医学版

碱性成纤维细胞生长因子预处理对大鼠脑缺血/再灌注损伤的神经保护作用

单位：东南大学医学院, 江苏南京 210009

分类号：R-33

出版年·卷·期（页码）：2015·34·第五期（714-720）

摘要：

目的:探讨碱性成纤维细胞生长因子(bFGF)预处理对脑缺血再灌注损伤(IR)的神经保护作用及其可能的机制。方法:90只雄性SD大鼠随机分成假手术组(A组)、脑缺血/再灌注对照组(B组)及bFGF预处理组(C组)。在制备脑缺血/再灌注模型前向C组大鼠侧脑室注射50 μg·ml^-1的bFGF 5 μl,向A组和B组大鼠侧脑室注射等体积的生理盐水,注射2 h后建立脑缺血/再灌注动物模型。分别于术后1、3、7 d进行改良的神经功能评分(mNSS),通过TTC染色计算梗死体积;术后1 d采用甲苯胺蓝染色和TUNEL染色技术观察缺血/再灌注区域神经细胞受损程度和凋亡情况。结果:与B组相比,C组mNSS降低,梗死体积缩小;细胞损伤程度减轻,TUNEL阳性细胞减少。结论:bFGF预处理对大鼠缺血再灌注损伤有神经保护作用,其机制可能与抑制缺血受损区域的神经细胞凋亡有关。

Objective:To explore the neuroprotective effect and the underlying mechanism of basic fibroblast growth factor(bFGF) pretreatment on cerebral ischemia-reperfusion injury. Methods: A total of 90 healthy male Sprague-Dawley(SD) rats were randomly assigned into three groups: group A (sham-operated group),group B(ischemia-reperfusion control group),and group C(bFGF pretreatment group). 5 μl of bFGF (50 mg·ml^-1 in saline) was administered to the animals of group C by intraventricular injection and an equal amount was administered to the animals of groups A and B by intraventricular injection.2 hours later, ischemia-reperfusion animal model was constructed.The loss of neurological function was assessed by the modified neurological severity score(mNSS) and the infarct volume was determined by TTC staining 1,3,and 7 days after MCAO. Toluidine blue staining(TBS) was performed for gross histological changes and TUNEL staining was performed for detection of cell apoptosis 1 day after MCAO. Results: In animals of group C,the mNSS score and the infarct volume both dropped; the extent of cell damage was alleviated and the number of TUNEL positive cells dropped compared with group B. Conclusion: bFGF pretreatment has a neuroprotective effect on cerebral ischemia-reperfusion injury in MCAO model of rat. The mechanism of pretreated bFGF in neuroprotection might be related to the inhibition of apoptosis of neurons within the ischemic region.

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