<em>ob/ob</em>与野生型小鼠脂肪组织的磁共振波谱研究-东南大学学报医学版

ob/ob与野生型小鼠脂肪组织的磁共振波谱研究

单位：东南大学附属中大医院放射科, 东南大学医学院分子与功能影像实验室, 江苏南京 210009

分类号：R329.3;R445.2

出版年·卷·期（页码）：2014·33·第三期（260-266）

摘要：

目的：探讨超高场强磁共振氢质子波谱（hydrogen MR spectroscopy，¹H-MRS）在全面评价ob/ob鼠及野生对照组小鼠白色脂肪组织和棕色脂肪脂质成分中的作用。方法：运用整体和单体素¹H-MRS方法对ob/ob及野生对照鼠进行活体波谱分析，整体¹H-MRS波谱定量全身脂质；单体素波谱计算白色和棕色脂肪的脂质含量，饱和及不饱和脂肪酸、双不饱和脂肪酸的相对含量及多聚不饱和程度。结果：整体¹H-MRS结果显示ob/ob鼠全身脂质含量显著高于野生型对照组，单体素¹H-MRS显示ob/ob鼠棕色脂肪脂质含量显著高于野生对照组，而两组鼠白色脂肪脂质含量的差异无统计学意义，ob/ob鼠白色脂肪的双不饱和脂肪酸及多聚不饱和程度均低于野生对照组。结论：¹H-MRS不仅可以定量白色和棕色脂肪的脂质含量，还可以分析其脂质成分。

Objective:To evaluate hydrogen MR spectroscopy(¹H-MRS) in estimating lipid content of whole body and measuring fat content in white and brown adipose tissue(WAT and BAT) of obese and normal mice in vivo.Methods:A single pulse ¹H-MRS and a localized proton ¹H-MRS were performed to measure the triglyceride from the whole body and the lipid contents in WAT and BAT of six ob/ob and six wild type(WT) mice using 7T micro-MR scanner. Fractions of unsaturated fatty acids(FU), diunsaturated fatty acids(FDU) and polyunsaturation degree(PUD) in WAT and BAT were calculated by using MRS. Independent-sample t test was performed in comparison. Results:In vivo total body spectroscopy showed the liquid lipid accumulation degree on the whole body in ob/ob mice was significantly higher than that of controls(P<0.001). In vivo, BAT fat content in ob/ob mice measured by MRS was significantly higher than that of WT mice(P<0.001), but no significant difference was found between ob/ob and WT mice in WAT fat content(P>0.05). PUD value and FDU of WAT in ob/ob mice were significantly lower than those of WT mice(P<0.05), but no significant difference was found between ob/ob and WT mice in FU of WAT and PUD value, FU, and FDU of BAT(P>0.05). Conclusion:¹H-MRS is efficient method in fat quantification at 7.0 T MR. In additions, MRS analyses PUD value and FU of WAT or BAT. They have the potential for further application quantization of lipid composition and lipometabolism in longitudinal studies.

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