小鼠下肢急性缺血模型的制作及影像评价-东南大学学报医学版

小鼠下肢急性缺血模型的制作及影像评价

单位：东南大学附属中大医院放射科, 东南大学医学院分子与功能影像实验室, 江苏南京 210009

分类号：R361.12;R33-33

出版年·卷·期（页码）：2014·33·第三期（254-259）

摘要：

目的：建立稳定有效的小鼠下肢缺血实验方法，为进一步活体研究下肢缺血性疾病提供动物模型。方法：电凝法制作裸鼠单侧下肢股动脉缺血模型，并经X线、Micro-CT、超声、磁共振成像、近红外激光及组织病理学全面评价模型。结果：缺血模型制作后，大体病理观察缺血侧下肢皮肤颜色苍白，X线及Micro-CT均显示股动脉起始处血管中断；彩色多普勒超声及近红外激光光谱仪证实缺血肌肉的血流量及血氧饱和度均显著下降；磁共振T₂加权成像显示缺血肌肉水肿呈高信号，弥散加权成像显示缺血肌肉水分子弥散受限。组织病理学显示缺血肌纤维明显肿胀，肌间隙变窄。结论：本实验建立电凝法小鼠下肢缺血模型，并证明该模型成功构建，为进一步治疗下肢缺血性疾病及机制研究提供基础。

Objective:To develop hindlimb ischemia model of mice for studying the mechanism and therapeutic methods of lower-extremity peripheral arterial disease.Methods:After mice right hindlimb ischemia with electrocoagulation, the two hindlimb was evaluated by macropathology, X-ray and micro-CT, ultrasound, magnetic resonance imaging, near-infrared spectroscopy and histopathology. Results:After ischemia, the skin color in right hindlimb turned pale after surgery induced injury. X-ray and micro-CT images were obtained to assess the termination of the proximal origin of the right femoral artery. Compared to the left control hindlimb, a significant less level of blood flow and oxygen satuation was observed by ultrasound and near-infrared spectroscopy. In T₂ weight imaging and diffusion weighted imaging, the presence of edema in the right ischemic muscle and the higher ADC value were observed compare to the control. Histological examination of the section of injured muscle showed that most of the muscle cells were round, and the extracellular space was tight. Conclusion:The mice hindlimb ischemic model was built successfully to further study the therapeutic methods of lower-extremity peripheral arterial disease.

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