茶多酚对高糖所致人肾小球系膜细胞活性氧产生的影响-东南大学学报医学版

茶多酚对高糖所致人肾小球系膜细胞活性氧产生的影响

单位：1.东南大学基础医学院病理学与病理生理学系,江苏南京,210009； 2.东南大学附属中大医院肾脏科,江苏南京,210009

分类号：R363, R285.5

出版年·卷·期（页码）：2003·22·第三期（151-154）

摘要：

目的:探讨高糖对人肾小球系膜细胞活性氧产生的影响以及茶多酚对其的干预作用.方法:应用比色法检测高糖作用后培养系膜细胞上清液中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)的活力和丙二醛(MDA)、超氧阴离子自由基(O2(-*))的水平,以及加入茶多酚后各指标的改变.结果:高糖可降低系膜细胞培养上清液中SOD、GSH-PX的活力,增加MDA和O2(-*)的含量;茶多酚可提高上清液中SOD和GSH-PX的活力,降低MDA和O2(-*)的含量.结论:高糖可导致人肾小球系膜细胞活性氧产生增多,而茶多酚可进行有效干预.

Objective To investigate the effect of high glucose on reactive oxygen species (ROS) in human mesangial cells and the intervention of tea polyphenols(TPs).Method Colorimetric assay was used to detect SOD、GSH?PX、MDA、O 2 -· in the supernatant of high glucose culture of human mesangial cells treated with or without TPs.Results SOD and GSH?PX were lowered,while MDA and O 2 -· increased in the supernatant of high glucose culture without TPs.However,SOD 〔( 32.507± 3.368)NU·ml -1 〕 increased with TPs compared with that only with high glucose,GSH?PX〔(56.233±2.808) NU· ml -1 〕increased as well,while MDA〔(3.280±0.149)μmol·L -1 〕and O -· 2〔(29.751±2.528)N·L -1 〕decreased.Conclusion High glucose contributes to an increase in ROS produced by human mesangial cells and TPs can better control high glucose induced oxidative stress.

参考文献：

[1] 王蓓, 邓红, 苏宁. 茶多酚对糖尿病大鼠活性氧表达的影响. 东南大学学报(医学版)2002(1). doi:10.3969/j.issn.1671-6264.2002.01.010
[2] Lin J K, LIANG Y C, LIN SHAN S Y. Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade. 1999(6). doi:10.1016/S0006-2952(99)00112-4
[3] 郑颜萍, 陈裕盛, 陈文. 茶多酚对慢性肾功能衰竭患者脂质过氧化损伤的影响. 中国中西医结合急救杂志1999(4)
[4] 李楠, 宋炳辉, 林勇. 茶多酚的研究进展, 1999(3)
[5] Ha H, KIM K H. Pathogenesis of diabetic nephropathy:the role of oxidative stress and protein kinase C. 1999(2-3). doi:10.1016/S0168-8227(99)00044-3
[6] Ceriello A, MERCURI F, MERCURI F. Defective intracellular antioxidant enzyme production in type 1 diabetic patients with nephropathy. 2000(12). doi:10.2337/diabetes.49.12.2170
[7] 阎红, 刘英莉, 李清钊. 茶多酚和抗坏血酸对染石英粉尘大鼠血抗氧化酶活性的影响. 卫生研究2002(1). doi:10.3969/j.issn.1000-8020.2002.01.025
[8] 黄星培, 王林, 周琼芳. 茶多酚对吸烟小鼠超氧化物歧化酶活性的影响. 中国职业医学2002(3). doi:10.3969/j.issn.1000-6486.2002.03.034
[9] LAL M A, KORNER A, Matsuo Y. Combined antioxidant and COMT inhibitor treatment reverses renal abnormalities in diabetic rats. 2000(8). doi:10.2337/diabetes.49.8.1381

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