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浅蓝菌素对人结肠癌裸鼠移植瘤bcl-2及bax基因表达水平的影响
作者:黄培林1 李俐2 朱世能3 陆世伦3 戴振声1 金月玲1 
单位:1.南京铁道医学院,病理学教研室,江苏,南京,210009; 2.南京市第一医院,病理科,江苏,南京,210006; 3.上海医科大学-病理学教研室,上海,200032
关键词:结肠肿瘤 脂肪酸合酶 浅蓝菌素 细胞凋亡 裸鼠 
分类号:R735.3+5, R730.5
出版年·卷·期(页码):2000·19·第一期(8-10)
摘要:

目的:通过观察人结肠癌LoVo细胞裸鼠移植瘤bcl-2及bax基因表达水平,探讨脂肪酸合酶抑制剂浅蓝菌素选择性的抑瘤途径和机制.方法:建立人结肠癌裸鼠移植瘤模型,浅蓝菌素每公斤体重160-mg腹腔注射,1次.d-1,连续10-d,第17天处死并解剖动物,瘤组织作免疫组织化学检测bcl-2及bax基因表达水平.结果:浅蓝菌素处理组结肠癌LoVo细胞移植瘤bcl-2基因蛋白表达率为68.5%;对照组bcl-2蛋白阳性表达率为 87.8%,阳性细胞弥漫分布.两组间有显著性差异(P<0.01).bax基因蛋白在浅蓝菌素组移植瘤组织阳性表达率为75.4%,主要呈强阳性表达;对照组阳性表达率为9.5%.两组间比较有统计学差异(P<0.01).结论:浅蓝菌素可使结肠癌LoVo细胞移植瘤的bcl-2基因表达下调,bax基因表达增强,提示bcl-2及bax基因在浅蓝菌素诱导的人结肠癌LoVo细胞凋亡中可能起着重要的调控作用.

Objective  To investigate the antitumor  mechanisms of cerulenin through  determination of  bcl?2 and bax oncoprotein in transplantated tumor of colonic carcinomas in nude mice.Methods  The expression rate of bcl?2 and bax oncoprotein in the transplantation tumor were detected by using SP immunohischemistry staining method.Results  The expression rate of bcl?2 oncoprotein were significantly lower in cerulenin?treated group than that of the tumor control group.The expression of bax was contrary to bcl?2 oncoprotein.Conclusion  The mechanism of cerulenin induced cell death might be correlated with apoptosis, and bcl?2 and bax gene might play an important role in regulating cerulenin?induced apoptosis.

参考文献:

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