高糖环境下肾小球系膜细胞和内皮细胞相互作用对活性氧产生的影响以及茶多酚的干预作用-东南大学学报医学版

高糖环境下肾小球系膜细胞和内皮细胞相互作用对活性氧产生的影响以及茶多酚的干预作用

单位：1.东南大学附属中大医院,儿科,江苏,南京,210009； 2.东南大学基础医学院,病理学与病理生理学系,江苏,南京,210009;东南大学,肾脏病研究所,江苏,南京,210009； 3.东南大学,肾脏病研究所,江苏,南京,210009

关键词：高糖活性氧糖尿病肾病细胞间相互作用系膜细胞内皮细胞

分类号：R363, R285.5

出版年·卷·期（页码）：2004·23·第三期（151-154）

摘要：

目的:探讨在高糖环境下肾小球系膜细胞与内皮细胞相互作用对活性氧产生的影响和茶多酚的干预作用.方法:系膜细胞和内皮细胞单独分别培养和共同培养,分正常对照组、高糖组及茶多酚干预组,培养0、12、36h后,用分光光度法检测培养液中丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性.结果:高糖共同培养时SOD活性比两者分别单独培养时更低(P<0.01);共同培养时茶多酚干预组的SOD活性显著高于高糖组(P<0.01).高糖共同培养时MDA含量较两种细胞单独培养明显增高,高于两种细胞单独培养的总和(P<0.01);共同培养时茶多酚干预组MDA含量低于高糖组(P<0.01).结论:(1)高糖环境下,系膜细胞和内皮细胞存在相互作用,这种作用能促进肾细胞活性氧的产生;(2)茶多酚通过干预活性氧的产生影响细胞间相互作用.

Objective To study the effect of high glucose and tea polyphenols(TPs) on the role of oxidative stress via the interaction of mesangial cells and endothelial cells.Methods The model of intercellular interaction between mesangial cells and endothelial cells of diabetic nephropathy was established and divided into 3 groups: control,high glucose and intervented with TPs.The mesangial cells and endothelial cells were co-cultured in RPMI?1640 with or without TPs in high glucose media up to 36 hours.The activity of SOD and the level of MDA were determined by spectrophotometry in the culture media.Results The activity of SOD of co-culture was markedly lower than that from the ordinary monolayer cell culture (P<0.01 ).The activity of SOD of co-culture intervented by TPs was much higher than that of high glucose group(P<0.01).The level of MDA of co-culture was higher than that of the ordinary monolayer cell culture(P<0.01).The level of MDA of the group intervented by TPs was lower than that of high glucose group(P<0.01).Conclusions (1) Exposed to high glucose,mesangial cells and endothelial cells can interact with each other to promote ROS,which would break the balance of oxidant state and accelerate the development of diabetic nephropathy.(2)The intercellular interaction between mesangial cells and endothelial cells may be intervened by TPs,which are able to eliminate the excess of ROS.

参考文献：

[1] Scott B C, BUTLER J, HALLIWELL B. Evaluation of the antioxidant action of ferulic acid and catechins. 1993(4). doi:10.3109/10715769309056512
[2] 杨秀伟. 四氧嘧啶性糖尿病与自由基, 1994(3)
[3] LAL M A, KORNER A, MATSUO Y. Combined antioxidant and COMT inhibitor treatment reverses renal abnormalities in diabetic rats. 2000(8). doi:10.2337/diabetes.49.8.1381
[4] Ceriello A, MOROCUTTI A, MERCURI F. Defective intracellular antioxidant enzyme production in type 1 diabetic patients with nephropathy. 2000(12). doi:10.2337/diabetes.49.12.2170
[5] Ha H, KIM K H. Pathogenesis of diabetic nephropathy:the role of oxidative stress and protein kinase-C. 1999(2-3). doi:10.1016/S0168-8227(99)00044-3
[6] Lin J K, LIANG Y C, LIN-SHIAU S Y. Cancer chemoprevention by tea polyphenols through mitotic signal transduction blockade. 1999(6). doi:10.1016/S0006-2952(99)00112-4
[7] Tournaire C, CROUX S, MAURETE M T. Antioxidant activity of flavonoids:efficiency of singlet oxygen (1 delta g) quenching, 1993(3)
[8] 郑颜萍, 陈裕盛, 陈文. 茶多酚对慢性肾功能衰竭患者脂质过氧化损伤的影响. 中西医结合实用临床急救1999(4)
[9] 王蓓, 邓红, 苏宁. 茶多酚对糖尿病大鼠活性氧表达的影响. 东南大学学报(医学版)2002(1). doi:10.3969/j.issn.1671-6264.2002.01.010
[10] 张彤, 邓红, 苏宁. 茶多酚对高糖所致人肾小球系膜细胞活性氧产生的影响. 东南大学学报(医学版)2003(3). doi:10.3969/j.issn.1671-6264.2003.03.004

服务与反馈：

【文章下载】【发表评论】【查看评论】【加入收藏】

提示：您还未登录，请登录！点此登录