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高度糖基化终产物对大鼠主动脉平滑肌细胞G-蛋白亚基基因表达的影响
作者:胡向阳1 黄晓明1 刘乃丰2 金辉2 
单位:1.东南大学医学院现代医学实验中心,江苏,南京,210009; 2.东南大学附属中大医院,心脏科,江苏,南京,210009
关键词:高度糖基化终产物 G-蛋白 基因表达 主动脉平滑肌细胞 大鼠 
分类号:R-33, Q786
出版年·卷·期(页码):2004·23·第三期(145-147)
摘要:

目的:研究高度糖基化终产物(AGEs)对G-蛋白亚基基因表达的影响,了解G-蛋白在AGEs介导的胞内信号转导途径中的作用.方法:利用半定量RT-PCR技术分析大鼠主动脉平滑肌细胞受AGEs作用后G-蛋白亚基Goα、GsαmRNA的改变.结果:AGEs在大鼠主动脉平滑肌细胞中上调Gsα的表达,但却使GoαmRNA水平显著下降.结论:G-蛋白或其某些亚基在AGEs介导的胞内信号转导途径和病理过程中可能起一定作用.

Objective  To study the effects of advanced glycosylation end products(AGEs) on the expression of G?protein subunit mRNA,and to evaluate the significance of G?protein in AGEs-mediated signal transduction pathways.Methods  Alternation of G?protein subunit mRNA level in cultured rat aortic smooth muscle cell(ASMC) after exposure to AGEs was determined by a semi-quantitative reverse-transcription polymerase chain reaction(RT?PCR).Results  The expression of Gsα subunit mRNA was up-regulated at least two folds than in the controls by AGEs in ASMC,whereas the levels of Goαsubunit mRNA declined significantly.Conclusion  Some G?protein subunits may be involved in the AGEs-mediated cellular signal transduction pathways and pathological processes.

参考文献:

[1] Brownlee M. Advanced protein glycosylation in diabetes and aging. 1995(). doi:10.1146/annurev.med.46.1.223
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[5] 李世云, 孙子林, 刘乃丰. AGEs-BSA对人肾系膜细胞分泌RANTES的影响初探. 东南大学学报(医学版)2003(6). doi:10.3969/j.issn.1671-6264.2003.06.001
[6] ZHOU O G, LIU N F, XIE P L. Expression of receptor for AGEs and inhibition of AGEP-induced cytosolic calcium elevation by diltiazem in cultured rat arotic smooth muscle cells, 1997(1)
[7] Liu N F, YAN J C, HUANG X M. Effects of advanced glycosylation end products on diacylglycerol signaling pathway in cultured rat aortic smooth muscle cells, 1999(1)  

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