β3-AR激动剂BRL35135、氯沙坦和二甲双胍对肥胖大鼠心肌UCP-2mRNA表达的影响-东南大学学报医学版

β3-AR激动剂BRL35135、氯沙坦和二甲双胍对肥胖大鼠心肌UCP-2mRNA表达的影响

单位：1.东南大学附属中大医院,神经内科,江苏,南京,21O009； 2.东南大学附属中大医院,心脏科,江苏,南京,210009

分类号：R542.2, R589.2, R965

出版年·卷·期（页码）：2005·24·第六期（401-405）

摘要：

目的:探讨β3-肾上腺素能受体激动剂BRL35135、氯沙坦和二甲双胍对肥胖大鼠心肌解偶联蛋白-2(UCP-2)mRNA表达及对胰岛素抵抗的影响.方法:以高脂饮食建立肥胖大鼠模型,分别予BRL35135(0.5 mg·kg-1·d-1)、氯沙坦(25mg·kg-1·d-1)和二甲双胍(300mg·kg-1·d-1)干预6周.取其心肌组织,采用RT-PCR法检测心肌组织中UCP-2 mRNA的表达水平;测定药物干预后大鼠血糖、血脂及胰岛素水平.结果:BRL35135和氯沙坦干预组UCP-2 mRNA表达增加(P＜0.001,P＜0.005);二甲双胍干预组UCP-2mRNA表达轻度增加,但与对照组无显著性差异(P＞0.05).BRL35135和二甲双胍干预组血脂、血糖和胰岛素水平下降;氯沙坦干预组血糖和胰岛素水平降低,而血脂水平无明显变化.结论:BRL35135和氯沙坦可以升高心肌中UCP-2mRNA的水平,延缓心肌肥厚进程.

Objective To explore the effects of β_3-adrenoceptor agonist BRL35135,losratan and metformin on expression of UCP?2 in myocardium and insulin resistance of obese rats.Methods The expression of UCP?2?mRNA was studied in myocardium of obese rats treated with three agents for six weeks respectively,and RT?PCR method was used to check the relative content of UCP?2 mRNA.Results The β_3-adrenoceptor agonist BRL35135(0.5?mg·kg^{-1}·d^{-1} orally)and Losratan(25?mg·kg^{-1}·d^{-1} orally)increased the expression of UCP?2 mRNA((P<)0.001,(P<)0.005).Metformin(300?mg·kg^{-1}·d^{-1} orally)had no effects on expression of UCP?2 mRNA((P>)(0.05)).BRL35135 combined with metformin decreased the level of blood sugar,lipidemia and insulin;losratan decreased the level of blood sugar and insulin,but did not change the level of lipidemia.Conclusion BRL35135 and Losratan increase the expression of UCP?2 mRNA,delay the proceeding of myocardium hypertrophy.

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