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糖基化终产物对人肾小球系膜细胞半乳糖凝集素-3基因表达的影响
作者:马婵娟1 孙子林1 金晖1 杨涛2 杨益宁1 
单位:1.东南大学附属中大医院,内分泌科,江苏,南京,210009; 2.江苏省人民医院,内分泌科,江苏,南京,210029
关键词:糖基化终产物 人肾小球系膜细胞 糖尿病肾病 半乳糖凝集素-3 基因表达 
分类号:Q786, R587.2
出版年·卷·期(页码):2006·25·第三期(174-177)
摘要:

目的:研究糖基化终产物(AGEs)对人肾小球系膜细胞半乳糖凝集素-3(galectin-3)表达的影响.方法:将培养的人肾小球系膜细胞(HRMC)用不同浓度(50、100、200及400mg·L-1)的AGEs干预24 h或同一浓度(200mg·L-1)的AGEs干预0、48、48及72 h,以无血清培养基(DMEM)和相应浓度的牛血清白蛋白(BSA)为对照.用RT-PCR检测细胞中半乳糖凝集素-3的mRNA水平.结果:不同浓度AGEs组HRMC的半乳糖凝集素-3 mRNA水平与对照组相比均有显著差异(P<0.01),且随着AGEs浓度的增高,半乳糖凝集素-3 mRNA水平也逐渐增高(P<0.05);AGEs干预HRMC 24、48、72 h,半乳糖凝集素-3mRNA水平与对照组相比有显著差异(P<0.05),且随着干预时间的延长,半乳糖凝集素-3 mRNA水平也逐渐增高(P<0.01).结论:AGEs以时间及剂量依赖的方式促进HRMC半乳糖凝集素-3 mRNA的表达.

Objective To study the effect of advanced glycation end products(AGEs) on the expression of galectin-3 in cultured human renal mesangial cells(HRMC).Methods The AGEs-BSA was prepared by incubating bovine serum albumin(BSA) with glucose.The cultured HRMC were intervened with the same concentration of BSA and AGEs-BSA(50,100,200,400mg· L^{-1}) for 24h or 200 mg· L^{-1} AGEs-BSA for 0,24,48 or 72h respectively.The mRNA expression of galectin-3 in HRMC were analyzed by RT-PCR.Results Compared with that in cells incubated with DMEM and BSA,the level of galectin-3 mRNA in cells incubated with 50,100 or 200,400mg· L^{-1}AGEs-BSA respectively for 24h((P<)0.01)or with 200mg· L^{-1}AGEsBSA for 0,24,48 or 72h respectively(P<0.05)increased significantly.Moreover,with the increased concentration of AGEs-BSA(P<0.05)or the expanded incubating time(P<(0.01)),the level of galectin-3 mRNA increased.Conclusion The AGEs-BSA can stimulate the expression of galectin-3 in cultured HRMC in a concentration-and time-dependent manner.

参考文献:

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