Objective To study the effect of advanced glycation end products(AGEs) on the expression of galectin-3 in cultured human renal mesangial cells(HRMC).Methods The AGEs-BSA was prepared by incubating bovine serum albumin(BSA) with glucose.The cultured HRMC were intervened with the same concentration of BSA and AGEs-BSA(50,100,200,400mg· L^{-1}) for 24h or 200 mg· L^{-1} AGEs-BSA for 0,24,48 or 72h respectively.The mRNA expression of galectin-3 in HRMC were analyzed by RT-PCR.Results Compared with that in cells incubated with DMEM and BSA,the level of galectin-3 mRNA in cells incubated with 50,100 or 200,400mg· L^{-1}AGEs-BSA respectively for 24h((P<)0.01)or with 200mg· L^{-1}AGEsBSA for 0,24,48 or 72h respectively(P<0.05)increased significantly.Moreover,with the increased concentration of AGEs-BSA(P<0.05)or the expanded incubating time(P<(0.01)),the level of galectin-3 mRNA increased.Conclusion The AGEs-BSA can stimulate the expression of galectin-3 in cultured HRMC in a concentration-and time-dependent manner. |