脱细胞骨骼肌支架与雪旺细胞构建体外人工神经模型的研究-东南大学学报医学版

脱细胞骨骼肌支架与雪旺细胞构建体外人工神经模型的研究

单位：东南大学附属中大医院,整形外科,江苏,南京,210009

分类号：R318.119, R622.3

出版年·卷·期（页码）：2006·25·第一期（12-16）

摘要：

目的:研究来源于自体骨骼肌组织的脱细胞细胞支架和自体雪旺细胞作为种子细胞的混合培养物作为一种神经桥接物的可能性.方法:将体外培养、扩增的雪旺细胞与用化学萃取制备且经过无菌多聚赖氨酸预处理的脱细胞骨骼肌支架进行混合培养2周,用扫描电子显微镜观察两者生长与附着情况.结果:雪旺细胞可与自体脱细胞骨骼肌支架良好附着,并在支架上增殖、迁移,形成类似Büngner带的雪旺细胞链.结论:将自体脱细胞骨骼肌支架与雪旺细胞混合培养而构建体外人工神经模型是可行的.

Objective To investigate the possibility of the mixed culture of detergent-extracted muscle scaffold originated from autologous muscle seeded with autologous Schwann cells used to be nervous graft.Methods Schwann cells were cultured and proliferated in vitro.Detergent-extracted muscle scaffold was prepared by using chemical extraction and pretreated by polymer of lynines,then they were mixedly cultured for two weeks.Their growth and adhesion were observed by scaning electron microscope.Result Schwann cells adhered well to the autologous detergent-extracted muscle scaffold,and then proliferated and migrated on the scaffold,finally formed a Schwann cells columns just like Büngner’s band.Conclusion The artificial nerve model of autologous detergent-extracted muscle scaffold seeded with Schwann cells in vitro is suitable to repair nerve defect.

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