Objectives To explore the relationship between the level of special immune reaction in female guinea-pigs immunized with conspecific crude inner ear antigens(CIEAgs) and the degree of its offspring ’s hearing loss,and at the same time,to explore the characteristics of inner ear histopathology and hearing dysfunction in the offsprings.Methods The female guinea-pigs were immunized by CIEAgs,and then copulated with male guinea-pigs and produced offspring.Before and after immunity,the female guinea-pig ’s special antibodies against inner ear antigens in serum were measured in series using ELISA method and the level of special cellular immune reaction by 3H-TdR-lymphocyte transform test.The hearing function of all female guinea-pigs and offspring were tested with electrocochleography.In the end,all animal ’s temporal bones histological slices were observed under light microscope.ResultsAfter immunized with inner ear antigens,special antibodies in serum and special cellular immune reaction rose in some female guinea-pigs.The level of humoral and cellular imunizty peaked at the middle and last stage of pregnancy.A positive-relativity between the threshold of offspring guinea-pigs and their mother ’s special antibodies level in serum or acoustic nerve compound active potential(cAP) threshold were found.There were nonlinear twice polynomial relationship between the threshold of offspring guinea-pigs and their mother ’s special cellular immune reaction.Conclusion The higher the female guinea-pig ’s antibodies level in serum and cAP threshold,the more serious and more often their offspring ’s hearing loss.Along with postnatal time prolonging,the offspring guinea-pig ’s hearing dysfunction do not evidently change.The endolymphatic hydrops happens in offspring guinea-pigs with hearing loss.