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哮喘大鼠孤束核内H3受体对呼吸道P物质样免疫反应物的影响
作者：董榕1 胡春梅2 王月涵1 
单位：1.东南大学基础医学院,生理学与药理学系,江苏,南京,210009； 2.南京市胸科医院,江苏,南京,210029
关键词：大鼠 孤束核 H3受体 哮喘 P物质 
分类号：R332
出版年·卷·期（页码）：2008·27·第六期（414-417）
摘要：

目的:探讨大鼠孤束核(NTS)内组胺H3受体对哮喘发作时呼吸道内神经源性炎症介质P物质(SP)含量的影响.方法:取健康雄性SD大鼠成功制备哮喘模型后诱发哮喘急性发作,采用中枢立体定位及核团微量注射技术,分组依次向NTS内微量注射无菌生理盐水、H3受体激动剂RMHA以及其拮抗剂Thio,利用肺内SP特异性抗血清、免疫组化法(SABC法)、葡萄糖氧化酶-DAB-镍染色和计算机图像分析等技术检测肺内SP的含量.结果:和对照组相比较,哮喘大鼠NTS内微量注射1 μgRMHA,肺内SP样免疫反应物(SPR-IL)阳性数量显著减少、阳性密度降低,差异有显著统计学意义(P＜0.01);如果在哮喘大鼠NTS内注射Thio 5 μg,则可拮抗RMHA的上述效应.结论:大鼠NTS内H3受体参与减少哮喘发作时神经源性炎症介质SP的释放,从而可能抑制哮喘发作.

Objective To investigate the effect of histamine H3 receptors in nucleus tractus solitarius(NTS) on substance P(SP) in airways of asthma rats. Methods Male Sprague-Dawley(SD) rats were intravenously sensitized with ovalbumin. The model rats,randomly divided into four groups,were placed on a stereotaxic frame,and then were inserted a tube into NTS according the rat brain atlas. When the rat were challenged with ovalbumin iv,NS,R-methylhistamine(RMHA,a H3 receptor receptors agonist) and Thioperamide(Thio,a H3 receptors antagonist) were injected into NTS respectively. SP was detected by immunohistochemistry SABC method and glucose-DAB-nickel technique respectively. The density of SP were quantitatively analyzed by computer image analysis system Results After RMHA were injected into NTS in the asthmatic rats,the number and density of positive fibers in the airways of asthmatic rats decreased significantly(P<0.01). These effects were blocked by pre-microinjecting thioperamide,a H3 receptors antagonist. Conclusion This study suggests that histamine H3 receptors in NTS can ameliorate rat’s asthma by inhibiting the release of SP in airways.
参考文献：

[1] 董榕, 张敏. 侧脑室注射R-α-甲基组胺对哮喘豚鼠呼吸道P物质样免疫反应物的影响. 中国组织化学与细胞化学杂志2003(2). doi:10.3870/j.issn.1004-1850.2003.02.011
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[4] DOREULEE N, YANOVSKY Y, FLAGMEYER I. Histamine H3-receptors depress synaptic transmission in the corticostriatal pathway. 2001(1). doi:10.1016/S0028-3908(0)00101-5
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[6] 董榕, 张敏, 刘保森. 中枢组胺H3受体对哮喘豚鼠呼吸运动的影响. 中国应用生理学杂志2006(1). doi:10.3969/j.issn.1000-6834.2006.01.025
[7] 胡春梅, 董榕. 大鼠孤束核内H3受体对哮喘发作时脑组织中Fos蛋白表达的影响. 东南大学学报(医学版)2005(6). doi:10.3969/j.issn.1671-6264.2005.06.004
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