VEGF-A及其受体VEGFR-1与子宫内膜癌生物学行为关系的探讨-东南大学学报医学版

VEGF-A及其受体VEGFR-1与子宫内膜癌生物学行为关系的探讨

单位：1.南京大学医学院附属鼓楼医院,妇产科,江苏,南京,210008； 2.东南大学基础医学院,生理学和药理学系,江苏,南京,210009

分类号：R737.33, Q344.13

出版年·卷·期（页码）：2008·27·第四期（275-278）

摘要：

目的:检测50例子宫内膜癌组织及癌旁正常内膜组织的VEGF-A和VEGFR-1的表达情况,探讨VEGF-A及其受体VEGFR-1是否与子宫内膜癌的侵袭与转移有关.方法: 采用RT-PCR技术对50例癌组织及癌旁正常组织进行VEGF-A和VEGFR-1 mRNA 半定量检测;采用Western blot进行其蛋白质表达的半定量检测.结果:癌组织VEGF-A 和VEGFR-1 mRNA表达水平及蛋白质水平均明显高于癌旁正常内膜组织;VEGF-A和VEGFR-1的高表达与子宫内膜癌肌层侵袭的深度有关,与淋巴结的转移无关;低分化癌组织VEGF-A的表达明显高于中、高分化,VEGFR-1的表达与细胞分化无关.结论: VEGF-A及其受体VEGFR-1的高表达可引起子宫内膜癌的肌层侵袭;癌分化越差,VEGF-A表达越高.

Objective To investigate the expression of VEGF-A and VEGFR-1,in 50 cases of endometrial carcinomas and to evaluate their correlation with invasion and metastases.Methods Semiquantitative reverse transcription polymerase chain reaction analysis was performed to assess VEGF-A mRNA expression in 50 cases of endometrial carcinomas.Protein level was determined by Western blot.Results Increased levels of VEGF-A and VEGFR-1 mRNA and protein were found in endometrial carcinoma tissues comared with adjecent normal endometrial tissues.Expression of VEGF-A and VEGFR-1 gene were statistically analyzed to be related with depth of invasion.Significantly increased levels of VEGF-A in adenocarcinoma with poorly differentiated and undifferentiated carcinoma compared with well-and moderately differentiated adenocarcinoma.Conclusions Highly expressed VEGF-A and VEGFR-1 may induce invasion of endometrial carcinomas into myometrium.The more poorly differentiated the level of the carcinoma,the higher the level of the expression of VEGF-A.

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