丹参酮ⅡA对高糖培养的大鼠血管平滑肌细胞p38MAPK信号通路的影响-东南大学学报医学版

丹参酮ⅡA对高糖培养的大鼠血管平滑肌细胞p38MAPK信号通路的影响

单位：东南大学,发育与疾病相关基因实验室,江苏,南京,210009

分类号：R587.1, R-33

出版年·卷·期（页码）：2008·27·第二期（130-133）

摘要：

目的:观察丹参酮ⅡA对高糖诱导的血管平滑肌细胞增殖的影响,分析该作用与氧化应激以及丝裂原活化蛋白激酶p38(p38 mitogen-activated protein kinases,p38 MAPK)信号通路的关系.方法:取第3代原代培养的大鼠血管平滑肌细胞建立高糖诱导细胞增殖模型,实验分为正常对照组、高糖组和丹参酮ⅡA组,BrdU掺入法测定血管平滑肌细胞的DNA合成水平;黄嘌呤氧化酶法、硫代巴比妥酸法分别测定细胞培养上清中超氧化物歧化酶(SOD)、丙二醛(MDA)含量;免疫印迹法(Western blot)检测磷酸化p38MAPK蛋白表达.结果:高糖诱导的血管平滑肌细胞呈快速增殖状态,在10～25 mmol·L-1间增殖效应呈剂量依赖性;丹参酮ⅡA能抑制高糖诱导的血管平滑肌细胞增殖,提高高糖培养的细胞中SOD水平,降低MDA含量,减少p-p38MAPK蛋白的表达.结论:丹参酮ⅡA抑制高糖诱导的血管平滑肌细胞增殖,这可能与其能减少血管平滑肌细胞中的氧自由基,抑制p38MAPK信号通路有关.

Objective To observe the effect of tanshinone ⅡA(TSN) on proliferation of vascular smooth muscle cell(VSMC) induced by high glucose and explore its relationship with oxidative stress and p38MAPK signal transduction pathway.Methods The arterial blood vessels of rats were separated carefully to culture VSMC by the tissue adherence method,and the third generation cells were employed for experiment.The cells were divided into normal control group,high glucose group and TSN group.DNA synthesis was analyzed by ELISA of BrdU.The activity of SOD and the contents of MDA in medium were measured with Xanthine oxidase method and TBA method.The expression levels of phosphorylated p38MAPK were detected by Western blot.Results The proliferation of VSMC induced by high glucose appeared fast,and the effect was in a dose-dependent manner in a concentration range of 10-25 mmol·L-1.TSN can restrain the proliferation of VSMC under the high glucose.And it also had an effect on the SOD and MDA in medium.The expression levels of phosphorylated p38MAPK of VSMC pretreated with TSN was lower than that of the VSMC stimulated by high glucose.Conclution TSN suppresses the proliferation of VSMC induced by high glucose,which is possiblely related with the suppression of oxygen free radical and p38MAPK signal transduction pathway.

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