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丹参酮IIA对腹膜透析大鼠模型伴发腹膜纤维化的预防干预作用
作者:张苗 蒋春明 邵秋媛 
单位:南京市鼓楼医院
关键词:腹膜纤维化 丹参酮IIA 转化生长因子-β1 结缔组织生长因子 
分类号:
出版年·卷·期(页码):2011·30·第六期(842-848)
摘要:

目的:探讨丹参酮IIA对腹膜透析动物模型发生腹膜纤维化的影响。方法:将40只大鼠随机分为四组(每组n = 10),每组每日分别用生理盐水、4.25%的葡萄糖腹膜透析液(PDF)、含50或100 mg / L丹参酮IIA 浓度的PDF腹腔注射。8周后,处死大鼠,并将其腹膜组织样本收集后进行观察分析。运用免疫组织化学检测转化生长因子-β1(TGF-β1)和结缔组织生长因子(CTGF)在壁层腹膜中的表达。同时通过逆转录聚合酶链反应、免疫印迹法对大网膜上TGF -β1和CTGF中mRNA和蛋白的表达进行检测。结果:丹参酮ⅡA明显抑制了4.25%的葡萄糖PDF引起的腹膜致密层的增厚和胶原沉积,减少了壁层腹膜和网膜中剂量依赖性的TGF-β1和CTGF的表达。结论:丹参酮IIA能减轻腹膜透析大鼠伴发的腹膜纤维化改变。

Objective:To investigate the effects of Tanshinone IIA on peritoneal fibrosis in a PD animal model. Methods: Forty rats were randomly divided into four groups (n=10 per group) that received daily intraperitoneal injection of saline, 4.25% glucose-based peritoneal dialysis fluid (PDF), or PDF along with 50 or 100 mg/L Tanshinone IIA. Eight weeks later, the rats were sacrificed and peritoneal tissue samples were collected for analysis. The expression of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) in parietal peritoneum was examined by immunohistochemistry. The mRNA and protein expression of TGF-β1 and CTGF in omentum was examined by reverse transcription polymerase chain reaction or Western blot. Results:Tanshinone IIA significantly suppressed submesothelial compact zone thickening and matrix accumulation induced by 4.25% glucose-based PDF. Tanshinone IIA also reduced TGF-β1 and CTGF expression in parietal peritoneum as well as in omentum in a dose-dependent manner. Conclusion:Tanshinone IIA prevented the progression of peritoneal fibrosis in this rat model. Tanshinone IIA may be a novel therapy for peritoneal fibrosis in patients undergoing long-term PD.

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