>
网站首页期刊介绍通知公告编 委 会投稿须知电子期刊广告合作联系我们
最新消息:
内毒素休克大鼠血清中TNF-α、皮质醇、LPS的测定及肾上腺的病理改变
作者:孙佳 夏海鸣 
单位:东南大学
关键词:内毒素休克 大鼠 LPS TNF-α 皮质醇 肾上腺 
分类号:
出版年·卷·期(页码):2011·30·第五期(728-732)
摘要:

【摘要】目的:建立内毒素休克大鼠模型,检测其血清中肿瘤坏死因子(TNF-α)、皮质醇、LPS的浓度,以及肾上腺组织中的LPS浓度,并于光镜下观察肾上腺组织的病理改变。方法: Wistar大鼠40只,随机分为正常对照组(n=10)和内毒素休克组(n=30),内毒素休克组采用腹腔注射适量D-氨基半乳糖(D-GalN)致敏后,经尾静脉注射休克剂量内毒素(LPS),观察平均动脉压(MAP)变化,以评定模型是否成功。ELISA法测定血清中TNF-α、血清及肾上腺组织中LPS浓度,电化学放光免疫分析法测定血清中皮质醇浓度,光镜下观察肾上腺组织的形态学改变。结果:注射休克剂量的LPS后,大鼠MAP迅速下降,回升至正常后逐渐缓慢下降并维持较低水平,其中19只大鼠符合休克标准,其血清中TNF-α、皮质醇、LPS均明显高于对照组(p<0.01),肾上腺组织中的LPS浓度也明显高于对照组(p<0.01),光镜下可见肾上腺组织有明显充血和水肿。结论: LPS、TNF-α参与了内毒素休克的发生、发展过程。休克大鼠的血清皮质醇浓度明显升高,并伴有肾上腺组织的病理改变。

Abstract: Objective: By producing the rat models with endotoxic shock, to detect serum TNF-α, corticosteroid , LPS level and adrenal glands tissue LPS level,and to observe histological changes of adrenal gland tissue with microscopy. Methods: Forty Wistar rats were randomly divided into two groups: normal control group (n=10), endotoxic shock group (n=30). Endotoxic shock group were sensitized by D-GalN by intraperitoneal injection, then given LPS by tail vein injection. Changes of mean arterial pressure (MAP) were observed to evaluate the animal model. Serum TNF-αconcentration, serum and adrenal glands tissue LPS concentrations were measured by enzyme-linked immunosorbent assay(ELISA). Serum corticosteroid concentration was detected by Electro-Chemiluminescence Immunoassay(ECLI). Histological alterations of adrenalglands tissue were studied with microscopy. Results: The MAP decreased obviously after injection of LPS, then recovered to normal, and decreased slowly, and kept at a low level. 19 rats corresponded shock standards, whose serum TNF-α, corticosteroid and LPS concentrations were significantly higher than those of the normal control group(p<0.01), and adrenal glands tissue LPS concentration was also significantly higher than that of the normal control group(p<0.05). Pathological changes of adrenal glands tissue presented congestion and dropsy. Conclusion: LPS、TNF-αinvolves genesis and development of endotoxic shock. Serum corticosteroid of endotoxic shock rats increases obviously, and the adrenal glands tissue presented pathological changes.

参考文献:
服务与反馈:
文章下载】【发表评论】【查看评论】【加入收藏
提示:您还未登录,请登录!点此登录
您是第 405873 位访问者


copyright ©《东南大学学报(医学版)》编辑部
联系电话:025-83272481 83272483
电子邮件:
bjb@pub.seu.edu.cn

苏ICP备09058364