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催产素预处理对胎鼠海马脑片缺氧无糖损伤后OTR、GABAARβ1表达的影响
作者:陆廷凤  
单位:就读于东南大学临床医学院
关键词:催产素 海马 缺氧无糖损伤 催产素受体 γ-氨基丁酸A受体β1亚基 
分类号:
出版年·卷·期(页码):2010·29·第四期(0-)
摘要:

目的:研究催产素预处理对胎鼠海马脑片缺氧无糖损伤后催产素受体(OTR)、γ-氨基丁酸A受体β1亚基(GABAARβ1)表达的影响,探讨OT神经保护的作用机制。方法:采用胎龄20天SD大鼠海马脑片,实验组经含10-6 mol/L OT人工脑脊液(ACSF)孵育预处理后行缺氧无糖损伤(OGD),对照组经正常ACSF孵育后直接行OGD各15min,SP免疫组化法半定量测定海马CA1区OTR、GABAARβ1表达变化及电镜观察神经元损伤情况。结果:实验组海马CA1区OTR、GABAARβ1均显著高于对照组(t=2.118,p=0.048;t=2.272,p=0.036)。对照组大量神经元受损,核固缩或碎裂,部分核膜溶解,实验组细胞核、粗面内质网、线粒体均较对照组有所改善,核膜较光滑,胞浆肿胀较对照组减轻。结论:OT上调胎鼠海马OTR、GABAARβ1表达,减少神经元变性坏死,增强大脑耐受缺氧损伤的能力,对胎儿中枢神经系统起保护作用。

Objective:To investigate the effects of oxytocin on changes of oxytocin receptor(OTR)、gamma-amimobutyric acid receptor A β1 subunit (GABAARβ1) expression in fetal rat hippocampus induced by oxygen and glucose deprived(OGD), and to investigate the mechanism of neuroprotective effects of oxytocin. Methods:Gestational age 20 days SD rat hippocampus slices in the experimental group underwent pretreatment incubation by containing 10-6mol/L OT artificial cerebrospinal fluid (ACSF), the control group were incubated with normal ACSF directly for oxygen and glucose deprivation injury (OGD) for 15min. SP immunohistochemistry was taken to semiquantitative determine hippocampus CA1 area OTR, GABAARβ1 expression and observe neuronal damage in electron microscopy. Results: The expression of OTR, GABAARβ1 were significantly higher in CA1 area of hippocampus (t = 2.118, p = 0.048; t = 2.272, p = 0.036). A large number of damaged neurons in the control group, nuclear condensation or fragmentation, some of the nuclear membrane dissolves in the experimental group; while nuclei, rough endoplasmic reticulum, mitochondria, were improved in the control group and smooth nuclear membrane, cytoplasmic swelling were eased. Conclusion: OT increases hippocampal OTR, GABAAR expression, decrease neuronal degeneration and necrosis, enhance the capacity of the brain tolerance to hypoxia, and play the protective effects on fetal nervous system.

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